(ISIS) Image
selected in vivo
spectroscopy is used as a localization sequence to provide complete
gradient controlled three-dimensional localization with a reduced number of sequence cycles, e.g. for in vivo 31P
spectroscopy.
The ISIS method generates three 180° pulses prior to a 90° pulse, after which the
free induction decay is recorded. Specific 180° pulses (
slice-
selective) are combined and the FID's added or subtracted to generate a
spectrum.
An advantage of the ISIS method is that the
magnetization (before the final 90° pulse) is predominantly along the z-axis and so T2 effects are relatively small. This explains the value of this technique for 31P data acquisition, because some phosphorus metabolites (e.g. ATP) have short T2 values.
A disadvantage is that eight
acquisitions are required to accomplish the spatial localization, therefore the sequence cannot be used for localized
shimming.
Another problem, because any variation between these data collections (for example, due to movement) will degrade these applications, can be solved by incorporating outer volume
suppression techniques such as OSIRIS (modified ISIS).