Nuclei can retain their magnetic
orientation through a chemical reaction. Thus, if RF radiation is supplied to the spins at a
frequency corresponding to the
chemical shift of the nuclei in one chemical state so as to produce
saturation or
inversion, and chemical reactions transform the nuclei into another chemical state with a different
chemical shift in a time short compared to the
relaxation time, the
NMR spectrum may show the effects of the
saturation or
inversion on the corresponding, unirradiated line in the
spectrum. This technique can be used to study reaction kinetics of suitable molecules.